Toxicopathologic Effect of Paraquat (PQ) on Adult Albino Male Testis against Efficiency of olive oil
شبكة المؤتمرات العربية
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| Title |
Toxicopathologic Effect of Paraquat (PQ) on Adult Albino Male Testis against Efficiency of olive oil
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| Creator |
A. Hussien, Duha
Mohammed Jawad, Zainab Jamal Ibrahim, Zainab Ismail |
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| Description |
This study aimed to investigate the effects of PQ and antioxidant (olive oil) against induced oxidative stress in rats. Male rats (n=48) were randomly divided into 4 groups which administrated orally and daily for six weeks as follows: T1: 3mg /Kg B.wt PQ only, T2: 3mg /Kg B.wt PQ & 2ml /Kg B.wt Olive oil Kg B.wt. T3: 2ml/Kg B.wt Olive oil and T4: distilled water as control group, respectively. After 2, 4 and 6 weeks the blood samples were collected for estimation the testosterone hormonelevelsandSemen samples for Sperms activity. Three animals from each group sacrificed for the histological study of testis. results showed significant increase of Testosterone level in T3at 2 and 4 weeks (15.05±0.34) (215.65±3.88) respectively, and there was significant increase of total sperms count (8.5±0.22) and sperms motility (95.52±0.84), decrease of sperm Abnormalities (3.19 ± 0.11) and sperms immotility ( 37.55 ± 0.44) in T3 as compared with control while a significant decrease of sperms total count (6.37±0.26) and Sperms Motility (85.05±1.21) in T1 and a significant decrease of Sperms total count (6.56±0.22) and Sperms Motility (68.24±1.39) in T2 and also The results showed a significant increase of Sperms Abnormalities (4.12±0.23) and Sperms Immotility (43.82±0.56) in T1 and also The results showed a significant increase of Sperms Abnormalities (5.10±0.11) and Sperms Immotility (57.60±2.33) T2 as compared with control group. The histological sections revealed significant lesions in testes. at (T1) group Testes showed deficiency of spermatogenesis summarized by necrosis of spermatocytes, necrotic debris with infiltration of inflammatory cells in some seminiferous tubules, while the epididymis showed vacuolar degeneration of tubules appear , desquamation of epithelia lining of tubules, lumen filled with debris degenerated cells, sloughed microcilia and inflammatory cells infiltration .At vas deference sloughing of the mucosal epithelial lining and stereocilia with congestion of blood vessels and infiltration of inflammatory cells mainly lymphocytes and macrophages were seen .In the other hands, (T2) group notice the testes loss spermatocytes left only with base spermatic cells in the lumen of seminiferous, mild thickening of the testicular capsule due to congestion of blood vessels and edema in the interstitial stroma between tubules, sever destruction of spermatogenesis (germ layer of seminiferous tubules )infiltrated with inflammatory cells, atrophied and shrinkage of tubules other rat show disaggregated and moving away from each other between the tubules, many atrophic necrotic seminiferous tubules replaced with hyalinized eosinophiolic material filled their lumen, few of them appeared calcified (blue-purple in color). germinal cells necrosis, especially in spermatogonia and Leydig cells had an abnormal fibroblast-like appearance It was concluded the paraquat effect on male reproductive system reveled from Sperms activity ,Testosterone concentration, Sperms total account, Sperms Abnormalities, Sperms Motility, Sperms Immotility and histopathological changes of different male reproductive organs including possibilities of having chronic testicular destruction which may lead to male infertility.While, daily consumption of Olive oil can decrease stress oxidative and alteration spermatogenesis in albino rats.Keywords: Paraquat, olive oil, histopathology, rat https://doi.org/10.24897/acn.64.68.426
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| Publisher |
شبكة المؤتمرات العربية
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| Contributor |
—
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| Date |
2019-08-26
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| Type |
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion مؤلَّف محكَّم |
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| Format |
application/pdf
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| Identifier |
http://proceedings.sriweb.org/akn/index.php/art/article/view/268
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| Source |
مؤتمرات الآداب والعلوم الانسانية والطبيعية; 2019: المؤتمر العلمي الدولي العاشر
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| Language |
ara
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| Relation |
http://proceedings.sriweb.org/akn/index.php/art/article/view/268/271
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| Rights |
الحقوق الفكرية (c) 2019 Duha A. Hussien, Zainab Jamal Mohammed Jawad, Zainab Ismail Ibrahim
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