Effect of Low Level Laser Therapy on the Sheep Ribs Xeno Graft in the Treatment of Rabbits Long Bone Fractures

الملخص

The aim of this study was to estimate the clinical, radiological, and histopathological effect of the low level laser therapy (LLLT) on sheep ribs xeno graft which used as bone replacement for the treatment of the femoral fractures in rabbits. 20  adults rabbits of  local breed were employed to removing about 1cm length of bone from the femoral bone surgically, and replaced the empty space with suitable size of sheep ribs xeno graft which fixed by intramedullary pinning .The animals were divided to ,control group  was of 10 rabbits lifted for normal healing processing ,with no laser irradiation post operation ( P.O.), while the treatment group of 10 rabbits exposed to single daily dosage of  continuous diode laser at four points of the lateral aspect of the femoral bone for 5 minutes at 72th interval for 14th days p.o. laser dosage (850 nm,148.4J/cm2).The experimental period 12 wks p.o.

The clinical observation revealed that the treatment group normally use the affected limb within 14^th to 21^th days p.o. while it remain after 21th days p.o in the control group, At the end of 12th wks p.o. after removing the intramedullary pins, the femoral bones re-fractured in the control groups, while it remain in the treatment group stable well fixed and not fracture.

The radiological finding showed, early periosteal reaction with new bone formation at the end of the 1st week p.o. in the treatment group which increase in volume and opacity in the 3^rd and 4^th weeks p.o. to form the bony bridge, while in the control group the periosteal reaction and the new bone formation appears at the end of 2nd weeks p.o., and the bony bridge formation occurs at the end of 6th weeks p.o.

The histopathological finding after 6th wk's p.o. in the treatment groups mature and wide trabecular bone formation. The control group showed thin immature trabecular bone, with large cavities of none organized Connective tissues. At the  end of 12th wks p.o.in the treatment groups lamellar bone formation surround the xeno graft, with partial bony incorporation, new blood vessels and osteocytes invade the bony device .compare with the  control groups the trabecula bone not yet all mature, and many empty lucana and havesan canal .

The conclusion is the irradiated sheep ribs xeno graft by LLLT, can be used to fill the femoral defect, promote healing, and well fixed and tolerate body weight, with no complication or body rejection.

https://doi.org/10.24897/acn.64.68.148