Investigation for Pectinase Pathogenicity & Activity of Mycological disease (Leaf Spot) in Solanum lycopersicum

Intesar Ali Mezeal, Maayad Salh Hussin


Pathogenic ability of many fungal  species qualified for nine of   genera were  recognized. (8) fungi   were optimistic and positively talented to contaminate leaves of  tomato seeming contamination indicators  for the disease (  leaf spot) . Alternaria citri Alternaria alternata , Alternaria tenuissima , Botrytis  cinerea , Botrytis squamosa  were extremely infectious  and formed this desease in most of infested leaves . Consequences protest this , in unhealthy plant life by Alternaria  citri  the chlorophyll was tremendously communicable presented   maximum ominously condensed in (1.16 - 0.18) (mg/g fresh leaves ) for chlorophyll( a, b)  individually associated in (2 - 1.50) (mg/g fresh leaves )  in  controller plants.  Alternaria  alternate also march exciting condensed for chlorophyll (a, b ) were ( 1. 89 - 0.98) (mg/g fresh leaves ) correspondingly. (6) species were divided to capabilities to products pectinase   by (cup-plate) technique. Totally mycological verified remained (pectinase ) inventors then through adaptable shades. (3 )species displayed in height pectinase inactivity include(Alternaria  citri, Alternaria tenuissima  and Alternaria alternaria)    were( 29, 26 and 23 mm) correspondingly, further species were create to be practical pectinase action include(Mycosphaerella tassiana, Botrytis squamosal , Botrytis cinerea). Pectinase   formed by Alternaria citri    increased by   increasing of   incubation dated exhibition its supreme afterwards (ten days )   of incubation, in (30°C ,  pH= 6). Among numerous carbon cradles shared definitely in the  media  , (pectin) shaped resolute pectinase structure through Alternaria  citri, whereas (peptone )was  greatest nitrogen foundation considerable measures to pectinase.



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