Estimation of Alkaline Phosphatase Enzymes Level in the Femoral Transverse Fractures Healing in Rabbits

الملخص

This project was designed to estimate alkaline phosphatase (ALP) enzymes level during fractures healing in rabbits .fourteen adult male rabbits were employed to induce transverse fractures in the femoral bone, and fixed with intramedullary pining. The evaluation was done by daily clinical observation, weekly radiography, histopathological examination, and weekly serum ALP enzymes measurment. The clinical observation revealed local inflammatory signs, loss appetite and difficult to walk two days p. o. then re-use the limb gradually next week's with normal physiological activity, the radiographic findings revealed new bone formation that visible at the end of 2^nd week p. o. which increase in volume and density next weeks until the boney bridge formation at the end of 4^rd week, the  radiological union created at the end of 6^th week p. o. , the histopathological examination showed osteoid deposition and immature trabecular bone formation with highly differentiated osteoblast cells at the end of the 2^nd week p. o. which converted to mature and lamellar bone formation at the end of the 4^th week ,while at the end of the 8^th and 10^th  wk p. o. increase  lamellar bone formation, with less of the osteoblast cells ,the serial mean serum ALP enzyme  measurement first week prior to the operation was 41.15± 3.57 C, which increased to 99.50±0.89 b end of 1^st week, and  96.00±1.48 b end of 2^nd week , reach 108.00±7.30 b end of 3^rd week ,and 213.00±0.68 a end of 4^thweek,192.00±23.55 a end of 6^th week,96.10±1.45 b end of 8^th week,96.10±18.07 b end of 10^th week . of the peak of ALP revealed significant increase at the end of 4^th and 6^th week p. o. at P˂ 0.05, compare with the other weeks, and the mean value of all week significant at P˂ 0.05 compare with the first week prior to operation .The conclusion serum ALP enzyme is a bio marker indictors of fractures healing stages, and have a positive correlation with the osteoblast cells activity and bone formation.

https://doi.org/10.24897/acn.64.68.403