Detection of blaKPC1 gene in Klebsiella pneumoniae isolated from Iraqi patients

Saade Abdalkareem Jasim, Najwa Shihab Ahmed, Mohammed Nadhir Maaroof


Some bacterial species are highly effective in destroying the carbapenem group through their production of Carbapenemases, which are associated with increased bacterial infections through their development of antibiotic resistance mechanisms. To achieve this goal, we collected 180 samples from different sources included (92 blood, 33 smears of wounds, 13 smears of burns and 42 sputum). The samples were taken from patients attended Ibn-Baladi Hospital as well as Yarmouk Teaching Hospital and some external laboratories Baghdad Governorate. Samples were diagnosed based on cultured characteristics on the MacConkey agar media as well as the EMB medium, microscopic examination by observing the diplococci-negative bacteria and biochemical tests. Then used the VITEK2 System to increase accuracy in the diagnostic process before DNA extraction. DNA was isolated from 84 samples, the purity and concentration of samples extracted was measured with Nanodrop. Using the PCR technique, the blaKPC1 gene was detected of the size of 61 base pairs using specialized gene primers.

Keyword: K. pneumoniae, Carbapenemases, Nanodrop, VITEK2, PCR, blaKPC1.

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